The Ultimate Guide To https://www.ceesdekker.net
The Ultimate Guide To https://www.ceesdekker.net
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Applying solitary-molecule optical and AFM imaging and power-spectroscopy methods, we seek to unravel the mechanistic components of the framework and dynamics of chromosomes. Our analysis interests include things like:
Human Rad51 filaments on double- and one-stranded DNA: correlating common and irregular forms with recombination operate.
Back in 1991, Sumio Iijima in the NEC Corporation had noted the synthesis of carbon nanotubes. Band construction calculations advised that these nanotubes could exhibit metallic band conduction. This might be a major benefit about the conducting polymers we experienced explored, which ended up, in truth, disordered semiconductors presenting just variable-array hopping conductivity.
Due to the fact 2000, Dekker has shifted the primary emphasis of his work in the direction of biophysics where by he scientific studies the Houses of one biomolecules and cells using the resources of nanotechnology. This modification of industry was driven by his fascination for your impressive performing of Organic molecular buildings, in addition to with the very long-time period perspective that many intriguing discoveries might be envisioned in this area.[5] Existing traces of investigation in his biophysics team are from the parts of:[six]
2012, discovery that nucleoid occlusion underlies the accuracy of bacterial mobile division; and initial at any time research of your dynamics DNA supercoils and the discovery of supercoil hopping
Microbiology has only existed for a century, because we started to examine micro-organisms far more severely. I assumed nanobiology was an correct time period, because all molecular Organic applicable interactions occur within the nanoscale: a protein is a handful of nanometres in size, DNA is two nm thick.’
We use nanofabricated microfluidic buildings to sample spatial boundaries for micro organism and biomimetic vesicles with reconstituted protein networks which evaluate the basic part of spatial confinement on the molecular and cellular level. Our projects involve the subsequent:
Dynamic ParB–DNA interactions initiate and maintain a partition condensate for bacterial chromosome segregation
In 1993, being a youthful and keen associate professor at Delft University of Know-how, I'd at first targeted the exploration endeavours of my team on measuring the electrical conductance of just one conducting-polymer molecule. Inside of two yrs, we experienced produced a cees dekker device where only one wire of the phthalocyaninepolysiloxane polymer was connected in between two closely spaced steel nanoelectrodes.
There was 1 difficulty however — we did not observe any measurable electrical conduction from The only polymer molecules. We concluded that at The only-molecule degree, conducting polymers have been in fact not these good conductors, which was somewhat disappointing. We So broadened our view to try to locate additional promising molecules.
Due to the fact 2000 he moved to single-molecule biophysics and nanobiology, with exploration from reports of DNA loop extrusion and supercoiling to DNA translocation via nanopores. Additional not too long ago his investigate has focused on researching chromatin construction and mobile division with bacteria on chip, when he can also be seeking to eventually Establish synthetic cells from The underside up.
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2005, discovery with the mechanism of DNA uncoiling by topoisomerase enzymes; discovery of extended-vary conformational adjustments in Mre11/DNA maintenance complexes; and very first pressure measurements on a DNA molecule within a nanopore
Good-point out nanopores have tested to generally be a surprisingly versatile probe for one-molecule Assessment of DNA. I'll explain several of our modern results – especially DNA knots – along with our attempts to broaden the capabilities of good-point out nanopores even even further, while in the route of single-protein detection, graphene nanopores, plasmonic nanopores, and DNA origami nanopores.